angrysurgeon
08-30-07, 13:38
What you need:
double destilled water (ddH2O)
cuppersulfate (CuSO4 x 5H2O)
Trisodiumcitrate x 2 H2O
Disodiumcarbonate (Na2CO3)
Dissolve dissolve 17.3g of CuSO4x5H2O in 100mL hot water. Then in a separate
container, pour up 800 mL of ddH2O and dissolve 173g of Trisodiumcitrate x 2H2O and add 100g of Na2CO3. Let the first solution cool and pour the second solution into the first solution. Dilute volume to 1 L.
This Solution A is stable.
Take 5 mL of urine and add 8 drops (0.4 mL) of Solution A, and place testtube in boiling water for 3 minutes. Immidately report colour of the mixture according to the following:
Blue to green with no precipitate = 0-0,1g of Glucose/dL
Green with a yellow precipitate = 0,3 g/dL
Olive green = 1,0 g/dL
Brownish orange = 1,5 g/dL
Brick red = 2,0 or more
Info from appendix of Tietz's clinical chemistry
double destilled water (ddH2O)
cuppersulfate (CuSO4 x 5H2O)
Trisodiumcitrate x 2 H2O
Disodiumcarbonate (Na2CO3)
Dissolve dissolve 17.3g of CuSO4x5H2O in 100mL hot water. Then in a separate
container, pour up 800 mL of ddH2O and dissolve 173g of Trisodiumcitrate x 2H2O and add 100g of Na2CO3. Let the first solution cool and pour the second solution into the first solution. Dilute volume to 1 L.
This Solution A is stable.
Take 5 mL of urine and add 8 drops (0.4 mL) of Solution A, and place testtube in boiling water for 3 minutes. Immidately report colour of the mixture according to the following:
Blue to green with no precipitate = 0-0,1g of Glucose/dL
Green with a yellow precipitate = 0,3 g/dL
Olive green = 1,0 g/dL
Brownish orange = 1,5 g/dL
Brick red = 2,0 or more
Info from appendix of Tietz's clinical chemistry